HyClone RPMI 1640 media: Liquid

Mammalian cell culture media PNT1A

Experiment
Mammalian cell culture media PNT1A
Product
HyClone RPMI 1640 media: Liquid from Cytiva
Manufacturer
Cytiva

Protocol tips

Protocol tips
Supplement the medium with 10% fetal bovine serum (FBS) (Hyclone, Waltham, MA, USA), penicillin and streptomycin (0.1 mg·mL−1)

Publication protocol

The PNT1A (immortalization of normal, adult, prostatic, epithelial cells), PC3 (androgen-independent) and 22RV1 (androgen-dependent) prostatic cancer cell lines were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA). The cells were cultured in a complete RPMI-1640 medium (Hyclone, Waltham, MA, USA) with 10% fetal bovine serum (FBS) (Hyclone, Waltham, MA, USA), supplemented with penicillin and streptomycin (0.1 mg·mL−1) at 37 °C and 5% CO2 in a humidified incubator.

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Papers

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Paper title
Anticarcinogenic Effect of Spices Due to Phenolic and Flavonoid Compounds—In Vitro Evaluation on Prostate Cells
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Manufacturer protocol

Download the product protocol from Cytiva for HyClone RPMI 1640 media: Liquid below.

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