DMEM–Dulbecco's Modified Eagle Medium

Mammalian cell culture media T47D

Experiment
Mammalian cell culture media T47D
Product
DMEM–Dulbecco's Modified Eagle Medium from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
supplemented with 50 U/ml penicillin, 50 μg/ml streptomycin (Mediatech/Corning Life Sciences, Manassas, VA, USA), and 10% FBS (Gemini Bio-Products, West Sacramento, CA, USA)

Publication protocol

Human breast cancer cell lines T47D (mdm2 SNP309 G/G, mutant p53 L194F) and MDA-MB-231 (mdm2 SNP309 T/G, mutant p53 R280K) were purchased from the American Type Culture Collection (www.atcc.org; Manassas, VA, USA). Cells were maintained at 5% CO2 in DMEM (Life Technologies, Carlsbad, CA, USA) with 50 U/ml penicillin, 50 μg/ml streptomycin (Mediatech/Corning Life Sciences, Manassas, VA, USA), and supplemented with 10% FBS (Gemini Bio-Products, West Sacramento, CA, USA) in a 37 °C humidified incubator. T47D cells generated with inducible MDM2 knockdown were described previously [6]. Constitutive MDM2 or MDMX knockdown cell lines were generated by retroviral infection with MLP.GFP vector (a generous gift from Scott Lowe) containing mir30 short hairpin RNA (shRNA)-expressing vector, mdm2 151656 shRNA, or mdmx 13023 shRNA. The mir30 shRNA inducible expressing vector has been used as a control for numerous previous high-impact studies [23, 24], and the only difference for the stable knockdown cell lines was a constitutively active promoter. Cell lines were generated and selected as previously described. All stable knockdown cell lines were used as selected pools.

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Papers

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Paper title
Context-dependent roles of MDMX (MDM4) and MDM2 in breast cancer proliferation and circulating tumor cells
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for DMEM–Dulbecco's Modified Eagle Medium below.

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