DULBECCO MEM MEDIUM

Mammalian cell culture media T98G

Experiment
Mammalian cell culture media T98G
Product
DULBECCO MEM MEDIUM from Vitrocell
Manufacturer
Vitrocell

Protocol tips

Protocol tips
supplemented with 10% FBS, 100 U/mL penicillin and 100 µg/mL streptomycin (sDMEM) containing 20 mM HEPES

Publication protocol

The human glioblastoma T98G and U-87MG cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured as previously described14. On the day of the experiment, the cells were trypsinized and resuspended (16–32 × 106 cells/mL; >95% viability) in DMEM supplemented with 10% FBS, 100 U/mL penicillin and 100 µg/mL streptomycin (sDMEM) containing 20 mM HEPES. For experiments testing the components of the medium (glucose 5.5 mM or 11 mM, glutamine, sodium bicarbonate and FBS), cells were resuspended in the corresponding experimental medium as described in the figure legends. Cell suspensions were maintained at room temperature (ca. 23 °C) and used within 2.5 h.

The data reported here are from experiments conducted over 18 months; as the cells were expanded from different frozen aliquots, and the components of the medium (e.g., FBS) were from different batches, absolute mean values of some variables can be expected to vary (e.g., maxOCR can oscillate up to 25%) more than the standard error of the mean when experiments performed a couple of months apart are compared. Nonetheless, we would emphasize that each experimental protocol was conducted within 2 to 4 weeks and that these variations were not observed; moreover, the effects of treatments were consistent throughout the whole study.

Full paper   Login or join for free to view the full paper.

Reviews

DULBECCO MEM MEDIUM from Vitrocell has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Mammalian cell culture media T98G using DULBECCO MEM MEDIUM from Vitrocell.

Paper title
High glycolytic activity of tumor cells leads to underestimation of electron transport system capacity when mitochondrial ATP synthase is inhibited
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Vitrocell for DULBECCO MEM MEDIUM below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Mammalian cell culture media T98G using DULBECCO MEM MEDIUM from Vitrocell. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms