DMEM–Dulbecco's Modified Eagle Medium

Mammalian cell culture media U251

Experiment
Mammalian cell culture media U251
Product
DMEM–Dulbecco's Modified Eagle Medium from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
supplemented with 10% (vol/vol) fetal bovine serum (FBS, Gibco)

Publication protocol

Human glioma cell lines (A172, U87-MG,
U251, T98G) were obtained from the American
Type Culture Collection (ATCC, Manassas, VA,
USA); all glioma cell lines and 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% (vol/vol) fetal bovine serum (FBS, Gibco). Human brain microvascular endothelial cells (HBMECs) were purchased from 3H Biomedical (Stockholm, Sweden) and cultured in EC medium supplemented with 5%
FBS and EC growth supplement. HBMECs at
passages 2-10 were used in the experiments as
described below. The routine culture was done
in a humidified incubator maintained at 37°C with 5% CO2
and 95% air.


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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for DMEM–Dulbecco's Modified Eagle Medium below.

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