Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),liquid

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	supplemented with 10% (v/v) fetal bovine serum (Welgene) and 1% penicillin/streptomycin (Life Technologies, Seoul, Korea).

Protocol tips
supplemented with 10% (v/v) fetal bovine serum (Welgene) and 1% penicillin/streptomycin (Life Technologies, Seoul, Korea).

Publication protocol

HSG, A253 and SGT cells originating from human salivary glands were cultured in Dulbecco’s modified Eagle’s medium (Welgene, Daegu, South Korea) supplemented with 10% (v/v) fetal bovine serum (Welgene) and 1% penicillin/streptomycin (Life Technologies, Seoul, Korea). Cells were propagated at 37 °C in a humidified atmosphere of 5% CO2. A253 cells were pre-incubated with 5-Aza-CdR for times ranging from 4 h to 1 day, before the day indicated for each experiment.

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Manufacturer protocol

Download the product protocol from Welgene for Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),liquid below.

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