Minimum Essential Medium Eagle

Mammalian cell culture media HSG cells

Experiment
Mammalian cell culture media HSG cells
Product
Minimum Essential Medium Eagle from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
supplemented with 10% fetal bovine serum (SAFC Biosciences, Kansas) and 1% penicillin/streptomycin (10,000 units/mL, 10 mg/mL)

Publication protocol

The human salivary gland (HSG) cell line [32] was generous gifts from NIRS. The HSG cell line has previously been used and described in preclinical radiobiologic experiments with the carbon-ion beams at NIRS. Moreover, the ridge filter in the instrument was designed so that the HSG cells were uniformly irradiated at the SOBP in the carbon-ion beam at NIRS [35]. Based on these prior experiments, the HSG cell line was used for these studies. The HSG cell was grown as a monolayer in Eagle’s Minimum Essential Medium (EMEM, Sigma-Aldrich Japan, Tokyo), supplemented with 10% fetal bovine serum (SAFC Biosciences, Kansas) and 1% penicillin/streptomycin (10,000 units/mL, 10 mg/mL) at 37°C in a 5% CO2 atmosphere. Prior to the cell uptake experiments, HSG cells were washed twice with PBS and isolated as a single-cell suspension by trypsinizing with a solution of 0.25% trypsin in PBS. After the cells were resuspended in EMEM medium, cell numbers were counted using a Coulter counter (Beckman Coulter, Tokyo) and the cell concentration was adjusted to the desired concentration.

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Amino acid transport system - A substrate predicts the therapeutic effects of particle radiotherapy
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Manufacturer protocol

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