Gibco™ RPMI 1640 Medium

Mammalian cell culture media DU145

Experiment
Mammalian cell culture media DU145
Product
Gibco™ RPMI 1640 Medium from Fisher Scientific
Manufacturer
Fisher Scientific

Protocol tips

Protocol tips
supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin (Invitrogen, Carlsbad, CA, USA)

Publication protocol

Human prostate cancer cell lines PC3 and DU145 were obtained from the American Type Culture Collection (Manassas, VA, USA). They were maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin (Invitrogen, Carlsbad, CA, USA). The paclitaxel-resistant cell lines PC3-TxR and DU145-TxR were kind gifts provided by Professor Atsushi Mizokami (Kanazawa University, Kanazawa, Japan) and were maintained in 10 nmol/L paclitaxel-contained RPMI-1640 medium. Cell morphology was obsevered under an inverted microscope (Olympus, Tokyo, Japan) at 200× magnification. Paclitaxel was purchased from Invitrogen. Antibodies against E-cadherin, Vimentin, Snail, β-catenin, Claudin-1, Notch-1, Notch-2, Notch-4, Akt, glycogen synthase kinase-3β (GSK-3β), phosphorylated GSK-3β (p-GSK-3β), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65 were purchased from Cell Signaling Technology (Beverly, MA, USA) and β-actin was from Sigma (St. Louis, MO, USA). Human E-cadherin-specific small interfering RNAs (siRNAs), control siRNA, and lentiviral E-cadherin and control vectors were purchased from Genepharma (Shanghai, China). The luciferase reporter lentiviral vector and luciferase-expressing cells were constructed in our own laboratory. The γ-secretase inhibitor (GSI, a Notch inhibitor) was purchased from Calbiochem (San Diego, CA, USA).

Full paper   Login or join for free to view the full paper.

Reviews

Gibco™ RPMI 1640 Medium from Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Mammalian cell culture media DU145 using Gibco™ RPMI 1640 Medium from Fisher Scientific.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Fisher Scientific for Gibco™ RPMI 1640 Medium below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Mammalian cell culture media DU145 using Gibco™ RPMI 1640 Medium from Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms