RPMI 1640, with L-Glutamine and 25mM HEPES

Mammalian cell culture media DU145

Experiment
Mammalian cell culture media DU145
Product
RPMI 1640, with L-Glutamine and 25mM HEPES from Lonza
Manufacturer
Lonza

Protocol tips

Protocol tips
RPMI-1640 (BE12-167F; Lonza) was supplemented with fetal bovine serum (10270106; Thermo Fisher Scientific), AB-serum (H4522; Sigma-Aldrich) where indicated, 100 U/ml penicillin, 100 mg/ml streptomycin (DE17-603E; Lonza), 2 mmol/l l-glutamine (BE17-605E; Lonza), 25 mmol/l HEPES and 1 mmol/l sodium pyruvate

Publication protocol

DU145 prostate cancer cells were obtained from the American Type Culture Collection and maintained in culture with regular passaging for less than 6 months. Authentication was carried out by the supplier using cytogenetic, isoenzymatic and DNA profile analysis. The HLA type of DU145 cells is HLA-A03/A33/B50/B57 (Welsh Blood Transfusion Service, Cardiff, UK). The cells were mycoplasma free and tested regularly using a MycoAlert Mycoplasma Detection Kit (LT07-418; Lonza). Irradiation was carried out using a 137Cs-source (with regular dosimetry quality assurance) at a rate of 0.627 Gy/min.

RPMI-1640 (BE12-167F; Lonza) was supplemented with fetal bovine serum (10270106; Thermo Fisher Scientific), AB-serum (H4522; Sigma-Aldrich) where indicated, 100 U/ml penicillin, 100 mg/ml streptomycin (DE17-603E; Lonza), 2 mmol/l l-glutamine (BE17-605E; Lonza), 25 mmol/l HEPES and 1 mmol/l sodium pyruvate (Sigma-Aldrich). FBS was depleted of bovine exosomes by pelleting at 100,000 g for 2 h. Lipopolysaccharide (LPS, L4391), ATP (A6419) and AMP-CP (CD73 inhibitor, M8386) were obtained from Sigma. PGE2, (2292), NECA (1691) and SCH58261 (adenosine A2A receptor inhibitor, 2270) were obtained from Tocris. PGE2-receptor inhibitors AH-6809 (EP2 inhibitor; CAY14050) and AH-23848 (EP4 inhibitor; CAY-19023) were obtained from Cayman Chemicals.

Full paper   Login or join for free to view the full paper.

Reviews

RPMI 1640, with L-Glutamine and 25mM HEPES from Lonza has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Mammalian cell culture media DU145 using RPMI 1640, with L-Glutamine and 25mM HEPES from Lonza .

Paper title
Dominant immunosuppression of dendritic cell function by prostate-cancer-derived exosomes
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Lonza for RPMI 1640, with L-Glutamine and 25mM HEPES below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Mammalian cell culture media DU145 using RPMI 1640, with L-Glutamine and 25mM HEPES from Lonza . Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms