DMEM–Dulbecco's Modified Eagle Medium

Mammalian cell culture media MDA-MB-468

Experiment
Mammalian cell culture media MDA-MB-468
Product
DMEM–Dulbecco's Modified Eagle Medium from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
supplemented with 10% fetal bovine serum (FBS; Biochrome, Berlin, Germany) and 1% penicillin/streptomycin (Gibco Life Technologies)

Publication protocol

The human breast cancer cell lines MDA-MB-231, MDA-MB-468, MCF-7, and T47D were obtained from ATCC (Manassas, VA, USA). MDA-MB-231, MCF-7, and T47D cells were cultured in DMEM/Ham´s F12 (Gibco Life Technologies, Darmstadt, Germany) and MDA-MB-468 cells in DMEM (Gibco Life Technologies, Darmstadt, Germany), supplemented with 10% fetal bovine serum (FBS; Biochrome, Berlin, Germany) and 1% penicillin/streptomycin (Gibco Life Technologies). Cells were grown in a humidified atmosphere of 95% air and 5% CO2. Short tandem repeat profiling of all used cell lines was performed in August 2017 at the DSMZ (German Collection of Microorganisms and Cell Cultures) to verify their genetic authenticity. MDA-MB-468 cells were newly acquired.

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Manufacturer protocol

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