Anti-RFP antibody

Autophagy assay cell type - L2/CCL149

Experiment
Autophagy assay cell type - L2/CCL149
Product
Anti-RFP antibody from Abcam
Manufacturer
Abcam

Protocol tips

Protocol tips
- Stain cells with Primary Ab at dilution-1:200 and incubate overnight at 4C.

Publication protocol

For immunohistochemistry in vitro, GFP-LC3/CCL149 cells growing on glass cover slips were fixed for 15min with 4% paraformaldehyde. After being incubated with 0.5% Triton X-100/PBS solution for 30 min and washed with PBS three times, the GFP-LC3/CCL149 cells were blocked with 3% hydrogen peroxide for 15 min and subsequently incubated overnight at 4 °C with the primary antibodies. Binding was visualized with the appropriate peroxidase-conjugated secondary antibodies (AR1022, ZSGB-BIO) for 20-30 min at 37 °C.


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Papers

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Manufacturer protocol

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