Bovine Brain Endothelial Cell Growth Medium

Mammalian cell culture media BBMVEC

Experiment
Mammalian cell culture media BBMVEC
Product
Bovine Brain Endothelial Cell Growth Medium from Cell Applications Inc
Manufacturer
Cell Applications Inc

Protocol tips

Upstream tips
Cells were transfected 4 hours prior to drug treatments and drug treatments were incubated for 24 h prior to hypoxic exposure in standard cell culture conditions (21% O2) then moved into hypoxic conditions (3% O2) for 24h.
Protocol tips
Cell cultures were placed in a special humidified hypoxic chamber for up to 48 h

Publication protocol

Bovine Brain microvascular endothelial cells (BMEC) were obtained from Cell Applications INC, (B841-25, San Diego, California) and cultured in endothelial cell basal media (Cell Applications, INC B819-500). For all experiments, BMEC were used from passages 4–8, grown to confluence, and cultured under standard cell culture conditions (21%, ‘normoxia’, 5% CO2) unless otherwise noted.

Cell cultures were placed in a special humidified hypoxic chamber for up to 48 h. Use of an antechamber ensured that once cells became hypoxic, they were never re-exposed to a normoxic environment. The chamber utilized a positive pressure system and was supplied with a gas mixture of 3% O2, 5% CO2, and balance of nitrogen. All relevant permeability tests and preparation for hydrogen peroxide (H2O2) production assays were conducted within the hypoxia chamber to avoid introduction of normoxia during data collection.

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Papers

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Nrf2 Activation: A potential strategy for the prevention of Acute Mountain Sickness
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Manufacturer protocol

Download the product protocol from Cell Applications Inc for Bovine Brain Endothelial Cell Growth Medium below.

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