Human MesoEndo Cell Growth Medium

Mammalian cell culture media HCtASMC

Experiment
Mammalian cell culture media HCtASMC
Product
Human MesoEndo Cell Growth Medium from Cell Applications Inc
Manufacturer
Cell Applications Inc

Protocol tips

Protocol tips
Cells were cultured up to passages 5 through 8 in subtype-specific growth medium (Cell Applications). Cells were seeded on 6-, 12-, or 24-well plates and treated or radiated at a density of 70% to 80%. Endothelial cells were cultured and seeded in gelatin-coated (Attachment Factor Protein 1X, Thermo Fisher Scientific) flasks and plates.

Publication protocol

Primary, proliferating human carotid artery smooth muscle cells (HCtASMCs) and human carotid artery endothelial cells (HCtAECs) (Cell Applications, San Diego, California) were cultured up to passages 5 through 8 in subtype-specific growth medium (Cell Applications). Cells were seeded on 6-, 12-, or 24-well plates and treated or radiated at a density of 70% to 80%. Endothelial cells were cultured and seeded in gelatin-coated (Attachment Factor Protein 1X, Thermo Fisher Scientific) flasks and plates.

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Papers

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Paper title
miR-29b Mediates the Chronic Inflammatory Response in Radiotherapy-Induced Vascular Disease
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Manufacturer protocol

Download the product protocol from Cell Applications Inc for Human MesoEndo Cell Growth Medium below.

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