Medium 254CF, Kit

Mammalian cell culture media HEM

Experiment
Mammalian cell culture media HEM
Product
Medium 254CF, Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
supplement M-254 medium with HMGS-2, penicillin (100 U/ml), gentamicin (10 µg/ml) and amphotericin B (0.25 µg/ml) and culture the cells at 37°C in 5% CO2.

Publication protocol

The normal human epidermal melanocytes (HEMaLP, Cascade Biologists) were grown according to the
manufacturer’s instruction. The cells were cultured in
M-254 medium supplemented with HMGS-2, penicillin (100 U/ml), gentamicin (10 µg/ml) and amphotericin B (0.25 µg/ml) at 37°C in 5% CO2. All experiments were performed using cells in the passages 5–7.

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Papers

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Paper title
Cytotoxic effect of lomefloxacin in culture of human epidermal melanocytes.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Medium 254CF, Kit below.

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