MGMTM-4 Melanocyte Growth Medium-4 BulletKitTM

Protocol tips

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	melanocyte growth media (MGM) supplemented with melanocyte growth factors (MGF) (Lonza Walkersville Inc, Walkersville, MD)

Protocol tips
melanocyte growth media (MGM) supplemented with melanocyte growth factors (MGF) (Lonza Walkersville Inc, Walkersville, MD)

Publication protocol

Human epidermal melanocytes (HEMn) are isolated from neonatal foreskin of African-American donors and cultured for experiments. Cells were grown in melanocyte growth media (MGM) supplemented with melanocyte growth factors (MGF) (Lonza Walkersville Inc, Walkersville, MD). For the experiments with UVB the media were replaced with phosphate buffered saline (PBS) prior the exposure. Cells in their third passage were used for the experiments and pretreated with melatonin or its derivatives 24 h before exposure to UVB after UVB exposure for indicated amount of time. It must be noted that melanocytes used for the experiments were darkly pigmented with dendritic morphology that was not affected by UVB doses of 25 and 50 mJ/cm2 during relatively short periods of incubation including 3 and 24 h after UVR treatment. UV transilluminator 2000 from Bio-RAD Laboratories was used for UVB irradiation following protocols and UV spectra reported previously

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Manufacturer protocol

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