D-MEM (High Glucose) with L-Glutamine and Phenol Red

Mammalian cell culture media HLF

Experiment
Mammalian cell culture media HLF
Product
D-MEM (High Glucose) with L-Glutamine and Phenol Red from Fujifilm Wako Chemicals Europe Gmbh
Manufacturer
Fujifilm Wako Chemicals Europe Gmbh

Protocol tips

Protocol tips
Cells were maintained in culture medium, DMEM (Wako, Tokyo, Japan) that was supplemented with 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), and 0.1 mg/ml streptomycin and 100 U/ml penicillin (Meiji Seika, Tokyo, Japan) at 37°C in a humidified atmosphere (5% CO2).
the human lung adenocarcinoma epithelial cell line, A549 (European Collection of Cell Cultures, Salisbury, United Kingdom) was used to measure TGF-β1–induced decreases in E-cadherin levels, a marker of epithelial cells, in the absence or presence of a designated agent.

Publication protocol

Human fetal lung fibroblasts, HFL-1 cells, and WI-38 cells were purchased from American Type Culture Collection (Manassas, VA, USA) and RIKEN Cell Bank (Tsukuba, Ibaraki, Japan), respectively. Cells were maintained in culture medium, DMEM (Wako, Tokyo, Japan) that was supplemented with 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), and 0.1 mg/ml streptomycin and 100 U/ml penicillin (Meiji Seika, Tokyo, Japan) at 37°C in a humidified atmosphere (5% CO2). In some experiments, the human lung adenocarcinoma epithelial cell line, A549 (European Collection of Cell Cultures, Salisbury, United Kingdom) was used to measure TGF-β1–induced decreases in E-cadherin levels, a marker of epithelial cells, in the absence or presence of a designated agent.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Mammalian cell culture media HLF using D-MEM (High Glucose) with L-Glutamine and Phenol Red from Fujifilm Wako Chemicals Europe Gmbh.

Paper title
Antifibrotic effects of cyclosporine A on TGF-β1-treated lung fibroblasts and lungs from bleomycin-treated mice: role of hypoxia-inducible factor-1α.
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Manufacturer protocol

Download the product protocol from Fujifilm Wako Chemicals Europe Gmbh for D-MEM (High Glucose) with L-Glutamine and Phenol Red below.

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