FBMTM Fibroblast Growth Basal Medium

Mammalian cell culture media HLF

Experiment
Mammalian cell culture media HLF
Product
FBMTM Fibroblast Growth Basal Medium from Lonza
Manufacturer
Lonza

Protocol tips

Protocol tips
NPM was sterilized by ultraviolet light to kill any possible pathogens in the air sample. NPM-protein corona (NPC) samples were prepared as described above and dissolved in the medium by sonication. HLFs (ATCC® PCS-201-013™) were routinely grown using FGM™ fibroblast growth media (Lonza, USA) in a humidified atmosphere of 95% air and 5% CO2 at 37 °C. Cells were suspended at 2 × 106 cells/ml and mixed with the NPC solutions. NPM concentrations of NPC-HLFs were fixed at 25, 50 and 100 μg/ml, respectively. To demonstrate the bioactivity of the protein corona, transforming growth factor beta 1 (Sigma, USA), NPC-vitamin C, pure serum and pure nanoscale PM2.5 (NPM) were also mixed with HLFs following the same procedure described above. The final exposure concentrations were 10 ng/ml (TGF-β1), 0.5 mg/ml (vitamin C) and 100 μg/ml (NPM).

Publication protocol

NPM was sterilized by ultraviolet light to kill any possible pathogens in the air sample. NPM-protein corona (NPC) samples were prepared as described above and dissolved in the medium by sonication. HLFs (ATCC® PCS-201-013™) were routinely grown using FGM™ fibroblast growth media (Lonza, USA) in a humidified atmosphere of 95% air and 5% CO2 at 37 °C. Cells were suspended at 2 × 106 cells/ml and mixed with the NPC solutions. NPM concentrations of NPC-HLFs were fixed at 25, 50 and 100 μg/ml, respectively. To demonstrate the bioactivity of the protein corona, transforming growth factor beta 1 (Sigma, USA), NPC-vitamin C, pure serum and pure nanoscale PM2.5 (NPM) were also mixed with HLFs following the same procedure described above. The final exposure concentrations were 10 ng/ml (TGF-β1), 0.5 mg/ml (vitamin C) and 100 μg/ml (NPM).

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Mammalian cell culture media HLF using FBMTM Fibroblast Growth Basal Medium from Lonza .

Paper title
Protein corona of airborne nanoscale PM2.5 induces aberrant proliferation of human lung fibroblasts based on a 3D organotypic culture
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Manufacturer protocol

Download the product protocol from Lonza for FBMTM Fibroblast Growth Basal Medium below.

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