PrimaPure Porcine Endothelial Cells Growth Medium

Mammalian cell culture media PAOEC

Experiment
Mammalian cell culture media PAOEC
Product
PrimaPure Porcine Endothelial Cells Growth Medium from Cell Applications Inc
Manufacturer
Cell Applications Inc

Protocol tips

Protocol tips
supplement the media with 5% fetal bovine serum in humidified air with 5% CO2 at 37 °C

Publication protocol

Bovine AECs and PAECs from the same animal were purchased from Cell Applications (San Diego, CA) and maintained in PrimaPure™ Bovine Endothelial Cell Growth Medium (BECGM) (Genlantis, San Diego, CA). Cells were incubated in a humidified controlled gas flow incubator (Forma Series II water jacketed CO2 incubator, Thermo Electron Corporation, Marietta, OH) containing 5% CO2 at 37°C. Cells were grown to confluency, used between passages 6–15, and passaged using trypsin-EDTA. Confluent AECs and PAECs were incubated in a modular incubator chamber (Billups-Rothenberg, Del Mar, CA) containing 5% CO2 and one of the following: 0%, 5%, or 13% oxygen balanced with nitrogen at 37°C. Cells for 21% oxygen treatment were incubated in a humidified controlled gas flow incubator (Forma Series II water jacketed CO2 incubator; Thermo Electron Corporation, Marietta, OH) containing 5% CO2 at 37°C for the time period indicated in the respective figure legends.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Mammalian cell culture media PAOEC using PrimaPure Porcine Endothelial Cells Growth Medium from Cell Applications Inc.

Paper title
Hypoxia-induced increases in glucose uptake do not cause oxidative injury or advanced glycation end-product (AGE) formation in vascular endothelial cells
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Manufacturer protocol

Download the product protocol from Cell Applications Inc for PrimaPure Porcine Endothelial Cells Growth Medium below.

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