BSK-H Medium for the cultivation of Borrelia spec.

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	supplemented with 6% heat-inactivated rabbit serum (Sigma-Aldrich).

Protocol tips
supplemented with 6% heat-inactivated rabbit serum (Sigma-Aldrich).

Publication protocol

B. burgdorferi strain B31 was grown at 33 °C until mid-exponential phase (5 × 107 cells/ml) in Barbour-Stoenner-Kelly medium (Bio & SELL) supplemented with 6% heat-inactivated rabbit serum (Sigma-Aldrich). Density of spirochetes was assessed employing dark field microscopy and a Kova counting chamber (Hycor Biomedical). Escherichia coli JM109 cells (Promega) used for cloning experiments and protein expression were grown at 37 °C in yeast tryptone broth, supplemented with the appropriate antibiotics. For complement inactivation assays, E. coli DH5α cells grown at 37 °C in yeast tryptone broth were employed.

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