BSK-H Medium, Complete

Protocol tips

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	-media includes 6% rabbit serum (complete media from Sigma-Aldrich -pH at room temperature 7.6 ± 0.3

Protocol tips
-media includes 6% rabbit serum (complete media from Sigma-Aldrich -pH at room temperature 7.6 ± 0.3

Publication protocol

Low passage isolates of two different Borrelia burgdorferi strains, B31 (ATCC # 35210) and 297 (ATCC # 53899), Borrelia afzelii (ATCC # 51992), and Borrelia garinii (ATCC # 51991) were obtained from American Type Tissue Collection. Escherichia coli DH5α strain was purchased from Life Technologies. Borrelia strains were maintained in complete BSK-H media, which includes 6% rabbit serum (complete media from Sigma-Aldrich #B8291) at 34oC with 5% CO2 and 100% humidity. Each new lot of complete BSK-H media was tested in a reference strain culture as a measure of quality assurance. Escherichia coli cells were maintained in standard Luria Broth media (Sigma) at 37oC in a shaking incubator.

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Manufacturer protocol

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