Chromogenic Culture Media

Bacterial cell culture media Clostridium difficile

Experiment
Bacterial cell culture media Clostridium difficile
Product
Chromogenic Culture Media from Biomerieux
Manufacturer
Biomerieux

Protocol tips

Protocol tips
CDIF media were supplied as pre-poured ready to use plates by bioMérieux (Craponne, France); each contained meat peptone (porcine) 8.0 g, taurocholate (bovine) 1.0 g, yeast extract 3.5 g, sodium chloride 6.0 g, selective mixture 0.27 g, chromogenic mixture 0.3 g, agar 13 g and purified water. TCCFA plates were supplied by PathWest Laboratory Medicine Excel Media and followed the original formulation of CCFA ( George et al., 1979 ) apart from reducing the antibiotic concentrations by 50 % (cycloserine 250 µg ml −1 and cefoxitin 8 µg ml −1) and including egg yolk at 60 ml l −1, plus the addition of 0.1 % synthetic sodium taurocholate (Sigma Cat # T4009), ρ-hydroxyphenylacetic acid at 1 g l −1 and agar at 13 g l −1. All plates were stored at 4 °C until used.

Publication protocol

CDIF media were supplied as pre-poured ready to use plates by bioMérieux (Craponne, France); each contained meat peptone (porcine) 8.0 g, taurocholate (bovine) 1.0 g, yeast extract 3.5 g, sodium chloride 6.0 g, selective mixture 0.27 g, chromogenic mixture 0.3 g, agar 13 g and purified water. TCCFA plates were supplied by PathWest Laboratory Medicine Excel Media and followed the original formulation of CCFA ( George et al., 1979 ) apart from reducing the antibiotic concentrations by 50 % (cycloserine 250 µg ml −1 and cefoxitin 8 µg ml −1) and including egg yolk at 60 ml l −1, plus the addition of 0.1 % synthetic sodium taurocholate (Sigma Cat # T4009), ρ-hydroxyphenylacetic acid at 1 g l −1 and agar at 13 g l −1. All plates were stored at 4 °C until used.

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Papers

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Paper title
Isolation of Clostridium difficile from faecal specimens--a comparison of chromID C. difficile agar and cycloserine-cefoxitin-fructose agar.
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Manufacturer protocol

Download the product protocol from Biomerieux for Chromogenic Culture Media below.

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