CardioTACS™ In Situ Apoptosis Detection Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not allow sample to dry at any stage prior to completion of protocol		If there is no staining in TACS Nuclease treated sample, Increase incubation time with Proteinase K (up to 60 minutes) or Cytonin™ (up to overnight at 4 oC and/or Optimize time for Nuclease treatment (5 minutes up to 2 hours).

Upstream tips
Do not allow sample to dry at any stage prior to completion of protocol
Downstream tips
If there is no staining in TACS Nuclease treated sample, Increase incubation time with Proteinase K (up to 60 minutes) or Cytonin™ (up to overnight at 4 oC and/or Optimize time for Nuclease treatment (5 minutes up to 2 hours).

Publication protocol

In SD rats, programmed cardiomyocyte cell death has been shown to affect the postnatal myocardium (Kajstura et al. 1995). This phenomenon was no longer detected by 21 days of age (Kajstura et al. 1995). In the present study, it was felt that both biochemical (apoptosis) and microscopic examination of cardiac morphology could help in identifying potential causes of age-related differences in cTnI concentrations. As a result, the terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling (TUNEL) assay was applied to sections of formalin-fixed, paraffin-embedded rat cardiac tissues. The procedure for detecting cardiac apoptosis is described elsewhere (Zhang et al. 2008). Apoptosis was detected with the CardioTACS in situ apoptosis detection kit (Trevigen, Inc., Gaithersburg, MD). Prepared myocardial sections were evaluated by light microscopy.

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Manufacturer protocol

Download the product protocol from Bio-Techne for CardioTACS™ In Situ Apoptosis Detection Kit below.

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