Trichloroacetic Acid Solution, MP Biomedicals™

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Previous isolation of membrane proteins with Gyros IncSupplier Diversity Partner REXXIP HN BUFFER 25 ML PER VI DFS Item (Fisher Scientific).	- Membrane proteins are precipitated by the addition of 8 volumes acetone (Fisher), 1 volume of a saturated trichloroacetic acid solution (Fisher). Incubation: −20°C for 45 min. After, are subjected to centrifugation (16,000 × g, 20 min, 4°C), rinsed in 80% cold acetone in distilled water, air dried, and solubilized.

Upstream tips
- Previous isolation of membrane proteins with Gyros IncSupplier Diversity Partner REXXIP HN BUFFER 25 ML PER VI DFS Item (Fisher Scientific).
Protocol tips
- Membrane proteins are precipitated by the addition of 8 volumes acetone (Fisher), 1 volume of a saturated trichloroacetic acid solution (Fisher). Incubation: −20°C for 45 min. After, are subjected to centrifugation (16,000 × g, 20 min, 4°C), rinsed in 80% cold acetone in distilled water, air dried, and solubilized.

Publication protocol

Prior to separation by using a 2D gel, membrane proteins were precipitated by the addition of 8 volumes acetone (Fisher), 1 volume of a saturated trichloroacetic acid solution (Fisher), and incubated at −20°C for 45 min. Precipitated protein samples were then subjected to centrifugation (16,000 × g, 20 min, 4°C), rinsed in 80% cold acetone in distilled water, air dried, and solubilized for isoelectric focusing by either 2DE-NEPHGE or IPGPhor pH 4-7 2DE-IPG strips (Amersham, Piscataway, NJ).

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