Cell Counting Kit-8

RNA quantification Coloremetric

Experiment
RNA quantification Coloremetric
Product
Cell Counting Kit-8 from Dojindo
Manufacturer
Dojindo

Protocol tips

Protocol tips
A549 and PC9 cells transfected with or without siRNA against circABCB10 and a FOXR2 overexpression vector were seeded into 96‐well plates at 5 × 103 cells/well and incubated for increasing durations (0, 12, 24, 48, and 72 hours) before adding 10 μL of Cell Counting Kit‐8 (CCK8) solution per well for 1 hour. Absorbance (450 nm) was measured using a Synergy microplate reader (BioTek, Winooski, VT).

Publication protocol

A549 and PC9 cells transfected with or without siRNA against circABCB10 and a FOXR2 overexpression vector were seeded into 96‐well plates at 5 × 103 cells/well and incubated for increasing durations (0, 12, 24, 48, and 72 hours) before adding 10 μL of Cell Counting Kit‐8 (CCK8) solution per well for 1 hour. Absorbance (450 nm) was measured using a Synergy microplate reader (BioTek, Winooski, VT).

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Papers

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Manufacturer protocol

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