Qproteome Bacterial Protein Prep Kit

Protein isolation Bacteria - Salmonella typhi

Experiment
Protein isolation Bacteria - Salmonella typhi
Product
Qproteome Bacterial Protein Prep Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- Protein isolation was performed following the manufacturer’s instructions.

Publication protocol

The cultures were grown until mid-exponential phase (OD600 of 2.7), and the 50 ml volume of cultures were used for a total protein extraction by using Qproteome Bacterial Protein Prep kit (Qiagen).

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Discussion

Discussion

2 years ago

Author: Hollie Fowler United Kingdom

How can I deal with my pellet being too viscous?

I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for Qproteome Bacterial Protein Prep Kit below.

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