Mouse ANGPTL3 ELISA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment. -Avoid repeated freeze-thaw cycles. -The kit may be stored at 4°C for up to 6 months. -Bring all reagents and samples to room temperature (18 - 25ºC) before use.	-Levels of Dkk-1 may vary between different samples. Optimal dilution factors for each sample must be determined by the investigator. -A standard curve must be run with each assay. -Keep substrate solution protected from light. -Read immediately after adding STOP solution.	-Store your standard at <-70ºC after reconstitution, others at 4ºC.

Upstream tips
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment. -Avoid repeated freeze-thaw cycles. -The kit may be stored at 4°C for up to 6 months. -Bring all reagents and samples to room temperature (18 - 25ºC) before use.
Protocol tips
-Levels of Dkk-1 may vary between different samples. Optimal dilution factors for each sample must be determined by the investigator. -A standard curve must be run with each assay. -Keep substrate solution protected from light. -Read immediately after adding STOP solution.
Downstream tips
-Store your standard at <-70ºC after reconstitution, others at 4ºC.

Publication protocol

Media were then collected, and the concentration of ANGPTL3 in conditioned media was determined using ANGPTL3 ELISA kits (mouse ANGPTL3: RayBiotech, catalog #ELM-ANGPTL3-1; human ANGPTL3: RayBiotech, catalog #ELH-ANGPTL3-1) following the manufacturer's instructions and using purified recombinant ANGPTL3 to generate a standard curve. The ELISA values for conditioned media prepared from untransfected cells were similar to blanks and this conditioned media was used as a control for all ANGPTL3 experiments.

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Manufacturer protocol

Download the product protocol from Raybiotech for Mouse ANGPTL3 ELISA below.

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