ApoAlert™ Annexin V-FITC Apoptosis Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 0.5-1 × 10^6 cells were cultured in a 6 welll plate - cells were pretreated with absence or presence of AZD8055 (500 nM). - Radiation was applied 4 h later.	- Annexin V and PI are added and incubated for 15 min at RT in the dark

Upstream tips
- 0.5-1 × 10^6 cells were cultured in a 6 welll plate - cells were pretreated with absence or presence of AZD8055 (500 nM). - Radiation was applied 4 h later.
Protocol tips
- Annexin V and PI are added and incubated for 15 min at RT in the dark

Publication protocol

Annexin V/PI Apoptosis Detection kit (Clontech Laboratories) was used for quantification of apoptosis. Cells were seeded in 6-well plates in the absence or presence of AZD8055 (500 nM), then radiation was applied 4 h later. After cultured for 24 h, 0.5-1 × 106 cells were collected into each tube and gently washed with PBS. Cell pellets were suspended in 1 × binding buffer and stained with Annexin V and PI. After incubated for 15 min at RT in the dark, the apoptosis analysis was carried out using a FACScan (BD Biosciences) and analyzed using FlowJo software (Tree Star Inc).

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Manufacturer protocol

Download the product protocol from Takara Bio Inc for ApoAlert™ Annexin V-FITC Apoptosis Kit below.

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