|- Prepare 1x Binding Buffer by diluting 1 ml of the 10x Binding Buffer with 9 ml of deionized water.
- Dissolve the staurosporine in DMSO to a concentration of 100 mg/ml.
- cells were pretreated with BER for 1 h, then cultured with or without fMLP (1µM) stimulation for 12 h
|- Apoptotic neutrophils are here defined as Annexin V-positive but propidium iodide-negative cells.|
Annexin V-FITC Apoptosis Detection Kit from Sigma-Aldrich has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Check out relevant papers found by Labettor's AI that are relevant for performing Apoptosis assay cell type - RAW 264.7 mouse macrophage using Annexin V-FITC Apoptosis Detection Kit from Sigma-Aldrich.
Download the product protocol from Sigma-Aldrich for Annexin V-FITC Apoptosis Detection Kit below.Download manufacturer protocol
Check out videos that might be relevant for performing Apoptosis assay cell type - RAW 264.7 mouse macrophage using Annexin V-FITC Apoptosis Detection Kit from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.