Mouse Prolactin ELISA

ELISA Mouse - PRL

Experiment
ELISA Mouse - PRL
Product
Mouse Prolactin ELISA from Raybiotech
Manufacturer
Raybiotech

Protocol tips

Upstream tips
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment.
-Avoid repeated freeze-thaw cycles.
-The kit may be stored at 4°C for up to 6 months.
-Bring all reagents and samples to room temperature (18 - 25ºC) before use.
Protocol tips
-Levels of Dkk-1 may vary between different samples.
Optimal dilution factors
for each sample must be determined by the investigator.
-A standard curve must be run with each assay.
-Keep substrate solution protected from light.
-Read immediately after adding STOP solution.
Downstream tips
-Store your standard at <-70ºC after reconstitution, others at 4ºC.

Publication protocol

Intra-cardiac blood was collected from mice on day 2 of lactation. Sera were collected after blood coagulation followed by a 10-minute centrifugation at 500 g. Serum prolactin (PRL) was quantified using the RayBio Mouse Prolactin ELISA kit according to the manufacturer’s instructions (RayBiotech).

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Papers

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Manufacturer protocol

Download the product protocol from Raybiotech for Mouse Prolactin ELISA below.

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