Cell Proliferation ELISA, BrdU

Cell cytotoxicity / Proliferation assay cell type - MDA-MB-231 breast adenocarcenoma

Experiment
Cell cytotoxicity / Proliferation assay cell type - MDA-MB-231 breast adenocarcenoma
Product
Cell Proliferation ELISA, BrdU from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
- Cells were seeded at a density of 1 × 104 cells/well of 96-well plates containing DMEM with 10% FBS
Protocol tips
- Cells were treated with BrdU solution 3h before the assay

Publication protocol

BC cells (MCF-7 and MDA-MB-231) were seeded at a density of 1 × 104 cells/well of 96-well plates containing DMEM with 10% FBS. After attachments, cells were treated with different doses of ASA or DMSO for different times. Cell proliferation was determined by BrdU–ELISAs (colorimetric) according to the manufacturer’s instructions (Roche Diagnostics, Indianapolis, IN, USA). Briefly, 3 hours before the indicated time of treatment, cells were treated with BrdU solution (10 μl/well). Following BrdU treatment, cells were incubated with FixDenat solution for 30 min and then incubated with BrdU POD for 90 min at room temperature. Cells were rinsed thoroughly with washing solution and incubated with substrate solution at room temperature and processed for photometric detection.

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Paper title
Aspirin blocks growth of breast tumor cells and tumor-initiating cells and induces reprogramming factors of mesenchymal to epithelial transition.
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Manufacturer protocol

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