TACS® 2 TdT Fluorescein Kit

TUNEL assay cell type - HeLa cells human cervical cancer

Experiment
TUNEL assay cell type - HeLa cells human cervical cancer
Product
TACS® 2 TdT Fluorescein Kit from Trevigen
Manufacturer
Trevigen

Protocol tips

Protocol tips
Fix cells in methanol for 10 min.

Incubate with 2% proteinase K solution for 15 minutes at room temperature.

Incubate with 50 μL of Labeling Reaction Mix at 37˚C for 1 hour in a humidity chamber.
Downstream tips
Analyze by fluorescence microscopy with a 495 nm filter.

Publication protocol

For the detection of neuronal apoptosis, the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) assay was performed in both Ishikawa and HeLa cells using a commercial kit (TACS® 2 TdT-Fluor Apoptosis Detection Kit) according to the manufacturer's instructions. Briefly, the cells were immersion hydrated, and the fixed and immobilized samples were rinsed with a PBS solution for 10 minutes. The samples were pre-incubated with a 2% proteinase K solution for 15 minutes at room temperature before being incubated in a TdT labeling buffer for 5 minutes and with a labeling reaction mix for 60 minutes in a humidity chamber. After the cells were washed with PBS (0.05%), they were incubated for 30 min in the dark with a Strep-Fluor solution. After an additional washing period, the tissues were covered with a DAPI mounting medium (Vector, #H-1200) and analyzed by fluorescence microscopy with a 495 nm filter.

Full paper   Login or join for free to view the full paper.

Reviews

TACS® 2 TdT Fluorescein Kit from Trevigen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

4 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - HeLa cells human cervical cancer using TACS® 2 TdT Fluorescein Kit from Trevigen.

Paper title
AFM-Detected Apoptotic Changes in Morphology and Biophysical Property Caused by Paclitaxel in Ishikawa and HeLa Cells
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Trevigen for TACS® 2 TdT Fluorescein Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing TUNEL assay cell type - HeLa cells human cervical cancer using TACS® 2 TdT Fluorescein Kit from Trevigen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms