|Fix cells in methanol for 10 min.
Incubate with 2% proteinase K solution for 15 minutes at room temperature.
Incubate with 50 μL of Labeling Reaction Mix at 37˚C for 1 hour in a humidity chamber.
|Analyze by fluorescence microscopy with a 495 nm filter.|
TACS® 2 TdT Fluorescein Kit from Trevigen has not yet been reviewed for this experiment
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2 years ago
Author: Ercole Udinesi
I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance
Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - HeLa cells human cervical cancer using TACS® 2 TdT Fluorescein Kit from Trevigen.
Download the product protocol from Trevigen for TACS® 2 TdT Fluorescein Kit below.Download manufacturer protocol
Check out videos that might be relevant for performing TUNEL assay cell type - HeLa cells human cervical cancer using TACS® 2 TdT Fluorescein Kit from Trevigen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
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