TdT In Situ Apoptosis Detection Kit - Fluorescein

TUNEL assay cell type - Rat fibroblast-like synoviocytes

Experiment
TUNEL assay cell type - Rat fibroblast-like synoviocytes
Product
TdT In Situ Apoptosis Detection Kit - Fluorescein from R&D system, Minneapolis, MN, USA
Manufacturer
R&D system, Minneapolis, MN, USA

Protocol tips

Protocol tips
Treat with Proteinase K Solution and incubate 15 to 30 minutes at room temperature, or cover
sample with 50 μL of Cytonin and incubate for 30-120
minutes at room temperature or 2-8 °C.

Treat with Labeling Reaction Mix and incubate at 37˚C for 1 hour in a humidity chamber.

Publication protocol

The terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) kit (R&D Systems Inc., Minneapolis, Minnesota, USA) was employed to measure the apoptosis rate of each group. FLS were seeded into a 6-well plate and stained according to the kit instructions. The cell apoptosis was observed under a light microscope, and cells with brown particles in their nucleus were regarded as positive cells. Five fields were selected for cells in each glass slide with high power microscopes (400×). The numbers of apoptotic positive cell nuclei and total cell nuclei were separately counted. The apoptotic index (AI) was determined by the ratio of the number of apoptotic positive cell nuclei to the number of total cell nuclei. This experiment was repeated three times and the mean value was obtained.

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Reviews

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Discussion

Discussion

1 year ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

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Manufacturer protocol

Download the product protocol from R&D system, Minneapolis, MN, USA for TdT In Situ Apoptosis Detection Kit - Fluorescein below.

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