Live and Dead Cell Assay (Abcam)

Live / Dead assay mammalian cells - L29 mouse fibroblast

Experiment
Live / Dead assay mammalian cells - L29 mouse fibroblast
Product
Live and Dead Cell Assay (Abcam) from Abcam
Manufacturer
Abcam

Protocol tips

Protocol tips
Add 4 μM of calcein-acetomethoxy (calcein AM-5 μL) and 2 mM of ethidium-bromide (20 μL).

Incubate this mixture for 30–45 min at room temperature in the dark

Publication protocol

Cell-drug interaction was also evaluated using cell viability assay (live-dead staining kit) over the period of 21 days. From the stock solution, 4 μM of calcein-acetomethoxy (calcein AM-5 μL) and 2 mM of ethidium-bromide (20 μL) were diluted in 10 mL PBS (tissue culture grade). 20–50 μL of working solution was added to each well and plates were incubated for 30–45 min and labeled cells were observed under Nikon fluorescent microscope as per general protocol [23].

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Papers

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Paper title
Effect of Extracts of on Proliferation of Keratinocytes and Fibroblasts Cells: An Alternative Approach for Wound Healing
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Manufacturer protocol

Download the product protocol from Abcam for Live and Dead Cell Assay (Abcam) below.

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