Live or Dead™ Cell Viability Assay Kit *Green/Red Dual Fluorescence

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 100 µL/well (96-well plate) or 25 µL/well of CytoCalcein™ Green/Propidium Iodide dye-working solution. Incubate the plate at room temperature or 37°C for 30 minutes to 1 hour, protected from light.	Monitor the fluorescence intensity with a fluorescence plate reader (bottom read mode) at Ex/Em = 490/525 nm

Protocol tips
Add 100 µL/well (96-well plate) or 25 µL/well of CytoCalcein™ Green/Propidium Iodide dye-working solution. Incubate the plate at room temperature or 37°C for 30 minutes to 1 hour, protected from light.
Downstream tips
Monitor the fluorescence intensity with a fluorescence plate reader (bottom read mode) at Ex/Em = 490/525 nm

Publication protocol

The different concentrations of EPO described above were added to a 96-well plate, in which EPCs were transferred at a cell density of 5×10 the cells/well. EPCs cultures were maintained for 7 days prior to staining. To identify the dead cells, calcein AM (AAT Bioquest, Sunnyvale, CA) and propidium iodide (BD Pharmingen, San Diego, CA) were used. A laser confocal microscope (Zeiss) was used to distinguish the live cells from the dead cells.


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Manufacturer protocol

Download the product protocol from AAT Bioquest for Live or Dead™ Cell Viability Assay Kit *Green/Red Dual Fluorescence below.

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