Anti-Sall4 antibody - ChIP Grade (ab29112)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Add protease inhibitors to all lysis solutions before use.	-Use at an assay dependent concentration. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.

Upstream tips
-Add protease inhibitors to all lysis solutions before use.
Protocol tips
-Use at an assay dependent concentration. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.

Publication protocol

ChIP was performed utilizing the EZ ChIP kit (Millipore) according to the manufacturer’s protocol. Immunoprecipitations were carried out with the following antibodies: IgG, anti-STAT3 (abcam, cat#ab76315), and anti-SALL4 (abcam, cat#ab29112). The primer sequences for SALL4 promoter are as follows: primer 1 forward 5´-GCCCA-GAGCAGTTATGGAAA-3´ and reverse 5´-ATTGA CACATGATGCCTGGA-3´; and primer 2 forward 5´-GATAGCTGGAGCAA GGATGG-3´ and reverse 5´-ATGAGCCCTGACAGCTGATT-3´. The primers for the miR-200c promoter are as follows: forward 5´-AGGGGTGAGACTAGGCAGGTTGG and reverse 5´-CCAGGTTGCAGTCCAAGCA.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ChIP Anti-bodies Sall4 using Anti-Sall4 antibody - ChIP Grade (ab29112) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Anti-Sall4 antibody - ChIP Grade (ab29112) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ChIP Anti-bodies Sall4 using Anti-Sall4 antibody - ChIP Grade (ab29112) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.