|re‐suspended cells in 0.9% NaCl containing 10 μg ml−1 nisin|
|Incubate both samples at room temperature for 1 hour, mixing every 15 minutes.|
|. Determine the optical density at 670 nm (OD670) for each bacterial suspension using a spectrophotometer.|
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1 year ago
Author: M. Daecher
Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?
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