Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Wash the cells twice with serum-free buffer or medium to remove serum esterase activity	Add calcein AM/EthD-III staining solution to cover the cell monolayerincubate the cells for 30-45 minutes at room temperature.

Upstream tips
Wash the cells twice with serum-free buffer or medium to remove serum esterase activity
Protocol tips
Add calcein AM/EthD-III staining solution to cover the cell monolayerincubate the cells for 30-45 minutes at room temperature.

Publication protocol

The heat-inactivated C. sporogenes bacteria were subjected to a bacterial viability test to confirm the effectiveness of the heat-inactivation process. The Viability/Cytotoxicity Assay kit for Bacterial Live & Dead Cells (Biotium, USA), was used to stain the IB using fluorescent nucleic acid dyes following the manufacturer’s protocol. DMAO is a green dye that stains both live and dead bacteria while Ethidium Homodimer-III (EthD-III) is a red dye that only stains dead bacteria. Images were obtained using a fluorescence microscope (Olympus, Japan).

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Manufacturer protocol

Download the product protocol from Biotium for Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells below.

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