Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells

Live / Dead assay bacteria - Clostridium sporogenes

Experiment
Live / Dead assay bacteria - Clostridium sporogenes
Product
Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells from Biotium
Manufacturer
Biotium

Protocol tips

Upstream tips
Wash the cells twice with serum-free buffer or medium to remove serum
esterase activity
Protocol tips
Add calcein AM/EthD-III staining solution to cover the cell monolayerincubate the cells for 30-45 minutes at room temperature.

Publication protocol

The heat-inactivated C. sporogenes bacteria were subjected to a bacterial viability test to confirm the effectiveness of the heat-inactivation process. The Viability/Cytotoxicity Assay kit for Bacterial Live & Dead Cells (Biotium, USA), was used to stain the IB using fluorescent nucleic acid dyes following the manufacturer’s protocol. DMAO is a green dye that stains both live and dead bacteria while Ethidium Homodimer-III (EthD-III) is a red dye that only stains dead bacteria. Images were obtained using a fluorescence microscope (Olympus, Japan).

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Discussion

Discussion

1 year ago

Author: M. Daecher Germany

Live/dead assay Bacteria

Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?

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Papers

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Manufacturer protocol

Download the product protocol from Biotium for Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells below.

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