ADAM10 was detected using a rabbit monoclonal antibody (GeneTex, EPR5622, 1:3,000). Human and murine PLEKHA7 was detected with previously described (28) rabbit polyclonal antibody against recombinant C-terminal fragments of PLEKHA7 (1:2,000 Western blot and 1:200 immunofluorescence). Canine PLEKHA7 was detected by Western blot with a polyclonal guinea pig antibody against recombinant C-terminal fragments of canine PLEKHA7 used at 1:1,000 (antibody a kind gift from Sandra Citi, University of Geneva, Geneva, Switzerland). Afadin was detected using a rabbit polyclonal antibody (Sigma, A0349, 1:2,000). N-cadherin was detected using a mouse monoclonal antibody (EMD Millipore, clone 13A9, 1;5,000). α-catenin was detected using a rabbit polyclonal antibody kindly provided by W. James Nelson, Stanford University, Stanford, CA, at 1:5,000. p38 MAPK was visualized with rabbit polyclonal antibody (Cell Signaling Technology, 9212, 1:1,000). Phosphorylated p38 MAPK (Thr180/Tyr182) was visualized using a rabbit polyclonal antibody (Cell Signaling Technology, 9211, 1:1,000). GAPDH was visualized with rabbit polyclonal antibody (Cell Signaling Technology, 5174, 1:5,000). S. aureus α-toxin was visualized with a rabbit antibody against H35L mutant α-toxin provided by Fabio Bagnoli at 1:1,000. Expression of FLAG-tagged PLEKHA7 constructs were visualized using rabbit polyclonal antibody anti-DYKDDDK (Cell Signaling Technology, 2368s, 1:2,500). Full paper
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