Total RNA Purification Kit

RNA isolation / purification Cells - immortalized CHO-K1

Experiment
RNA isolation / purification Cells - immortalized CHO-K1
Product
Total RNA Purification Kit from Norgen Biotek
Manufacturer
Norgen Biotek

Protocol tips

Upstream tips
- For lysis/cell disruption check the protocol beforehand to see which reagents/lab materials are needed.
Downstream tips
- Minimal DNA is left over using this kit, however if DNA is undesired an additional RNase free DNase treatment is warranted.

Publication protocol

Total RNA purification kit (without DNase treatment; Norgen, Canada) and RNeasy mini kit (with DNase treatment; Qiagen, USA) respectively. The extracted RNA was then reverse transcribed, and the cDNA was subjected to PCR-amplifying 18S.

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Reviews

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Discussion

Discussion

4 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized CHO-K1 using Total RNA Purification Kit from Norgen Biotek.

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Manufacturer protocol

Download the product protocol from Norgen Biotek for Total RNA Purification Kit below.

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Videos

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