SIRT1 siRNA and shRNA Plasmids (h)

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	Human PC-3 cells were provided by Antonio Filippini (Sapienza University), Cells were cultured in RPMI 1640 medium (Euroclone) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% antibiotics (Euroclone). For siRNA experiments, PC-3 cells were transfected with 40 pmol of human Sirt1 or with control siRNA purchased from Santa Cruz Biotechnology diluted in Opti-MEM (Gibco), using Lipofectamine RNAiMax as indicated by the manufacturer’s instructions. PC-3 cells were incubated for 48 h and then infected with VSVΔM51 at the indicated MOI.

Protocol tips

Human PC-3 cells were provided by Antonio Filippini (Sapienza University), Cells were cultured in RPMI 1640 medium (Euroclone) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% antibiotics (Euroclone). For siRNA experiments, PC-3 cells were transfected with 40 pmol of human Sirt1 or with control siRNA purchased from Santa Cruz Biotechnology diluted in Opti-MEM (Gibco), using Lipofectamine RNAiMax as indicated by the manufacturer’s instructions. PC-3 cells were incubated for 48 h and then infected with VSVΔM51 at the indicated MOI.

Publication protocol

Human PC-3 cells were provided by Antonio Filippini (Sapienza University), Cells were cultured in RPMI 1640 medium (Euroclone) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% antibiotics (Euroclone). For siRNA experiments, PC-3 cells were transfected with 40 pmol of human Sirt1 or with control siRNA purchased from Santa Cruz Biotechnology diluted in Opti-MEM (Gibco), using Lipofectamine RNAiMax as indicated by the manufacturer’s instructions. PC-3 cells were incubated for 48 h and then infected with VSVΔM51 at the indicated MOI.

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for SIRT1 siRNA and shRNA Plasmids (h) below.

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