FlashTag™ Biotin HSR RNA Labeling Kits

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		Label all samples at one time to avoid day-to-day variations. The labeled samples can be stored at -80°C upto 3 months and successive hybridizations

Downstream tips
Label all samples at one time to avoid day-to-day variations. The labeled samples can be stored at -80°C upto 3 months and successive hybridizations

Publication protocol

Expression profiling and statistical analysis
Total RNA was extracted from skin tissue and processed as previously described using the Flash Tag Biotin HSR RNA labeling kit and hybridized with the miRNA expression profiling GeneChip miRNA 2.0 Array according to the manufacturer’s protocol [41]. Raw data was pre-processed using R packages and normalized using RMA (Robust Multi-array analysis) to generate expression levels across the samples following instruction of at bioconductors.org [42].

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for FlashTag™ Biotin HSR RNA Labeling Kits below.

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