Live and Dead Cell Assay (Abcam)

Live / Dead assay yeast - Syspastospora parasitica

Experiment
Live / Dead assay yeast - Syspastospora parasitica
Product
Live and Dead Cell Assay (Abcam) from Abcam
Manufacturer
Abcam

Protocol tips

Protocol tips
Add 1X Live and Dead Dye to cells.

Incubate 10 min at room temperature

Publication protocol

The anti-oomycete assays were adjusted to the characteristics and life-cycles of the oomycete species. Zoospores of S. parasitica and S. diclina were adjusted to 10000 cells/ml and exposed to increasing concentrations (5, 10, 30, 100 µg/ml final concentration) of proteins. Because of the rapid encystment of zoospores into cysts, assessment of mortality was only performed at 30 min (prior to encystment of live zoospores). Zoospores of Phytophthora parasitica were adjusted to 500000 cells/ml and exposed to identical concentrations of proteins (5, 10, 30, 100 ug/ml final concentration). Microscopic observations of the number of live and dead zoospores were performed after 10, 30, 60, 120, 240 min of treatment. Zoospore-treated suspensions were stained immediately after incubation with the Live/Dead Cell Assay kit (Abcam) as described in the manufacturer's protocol. All conditions were tested in triplicate and assays were performed 3 times independently.

Full paper   Login or join for free to view the full paper.

Reviews

Live and Dead Cell Assay (Abcam) from Abcam has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay yeast - Syspastospora parasitica using Live and Dead Cell Assay (Abcam) from Abcam.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Abcam for Live and Dead Cell Assay (Abcam) below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Live / Dead assay yeast - Syspastospora parasitica using Live and Dead Cell Assay (Abcam) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms