Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Protocol tips

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	Dilute Gal Screen substrate with Gal Screen Buffer. Add to samples and incubate at 26-28C for 60-90 min

Protocol tips
Dilute Gal Screen substrate with Gal Screen Buffer. Add to samples and incubate at 26-28C for 60-90 min

Publication protocol

Next, we replaced the hrGFP gene with a β-galactosidase reporter gene for two of the plasmids (containing the promoters P-POT1 and P-CDC2 upstream of CEN) to test the generalizability of this approach. Both plasmids showed a similar improvement of roughly 50% in the β-galactosidase activity as seen with the hrGFP (Fig. 1c). In addition, the increase in the expression is preserved when a lower strength native promoter (P-EXP) is used to drive the expression of β-galactosidase, thus indicating that the approach is independent of reporter protein and promoter driving the expression cassette (Fig. 1d).

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Manufacturer protocol

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