FITC Annexin V Apoptosis Detection Kit I (RUO)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Publication protocol

To assess apoptosis in L02 cells, the level of Annexin V staining was determined by flow cytometry. After incubation, cells were treated according to the manufacturer's protocol (FITC Annexin V Apoptosis Detection Kit, BD Biosciences Pharmingen, San Diego, CA, USA) and analyzed by flow cytometry (FACSort, Becton Dickinson, Franklin Lakes, NJ, USA).

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Manufacturer protocol

Download the product protocol from BD Biosciences for FITC Annexin V Apoptosis Detection Kit I (RUO) below.

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