PE Annexin V Apoptosis Detection Kit I

Apoptosis assay cell type - PC-3

Experiment
Apoptosis assay cell type - PC-3
Product
PE Annexin V Apoptosis Detection Kit I from BD Biosciences
Manufacturer
BD Biosciences

Protocol tips

Upstream tips
- Cells treated with sorafenib (0–4 μM) alone or in combination with other agent.
Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Publication protocol

Cells were seeded onto six wells and treated with sorafenib (0–4 μM) alone or in combination as described above. After 48 h, the cells were harvested and centrifuged. Apoptosis was measured by using the PE Annexin V Apoptosis Detection Kit I in combination with flow cytometry (Becton Dickinson, Schwechat, Austria) according to the manufacturer's protocols. Assays for caspase 3/7 activity were performed with the Caspase-Glo 3/7 assay kit (Promega) according to the manufacturer's protocols (Santer et al. 2011).

Full paper   Login or join for free to view the full paper.

Reviews

PE Annexin V Apoptosis Detection Kit I from BD Biosciences has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Apoptosis assay cell type - PC-3 using PE Annexin V Apoptosis Detection Kit I from BD Biosciences.

Paper title
Sorafenib decreases proliferation and induces apoptosis of prostate cancer cells by inhibition of the androgen receptor and Akt signaling pathways
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from BD Biosciences for PE Annexin V Apoptosis Detection Kit I below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Apoptosis assay cell type - PC-3 using PE Annexin V Apoptosis Detection Kit I from BD Biosciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms