Cell Death Detection ELISAPLUS

Apoptosis assay cell type - CaOV-3

Experiment
Apoptosis assay cell type - CaOV-3
Product
Cell Death Detection ELISAPLUS from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
- 10,000 cells per well were platted for an assay

- Cells were pre treted with ABC294640, paclitaxel, or vehicle for 24 h.
Downstream tips
- When the absorbance of
samples too high,reduce agent-concentration and decrease incubation period.

- When the absorbance of
samples too low, increase the agent concentration and prolong the agent incubation interval

Publication protocol

Caov-3 cells were plated at 10,000 cells per well in DMEM and allowed to adhere overnight. Cells were then treated with ABC294640, paclitaxel, or vehicle for 24 h. A Cell Death Detection ELISA PLUS Kit from Roche Applied Science (Indianapolis, IN, USA) was used to process cells. Briefly, cells were lysed and transferred to an anti-nucleosome-coated microplate, incubated with colorimetric detection reagent, and the absorbance was read on a MicroTek plate reader at 405 nm.

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Papers

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Paper title
Targeting ovarian cancer and chemoresistance through selective inhibition of sphingosine kinase-2 with ABC294640.
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Cell Death Detection ELISAPLUS below.

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