|Synthetic siRNAs targeting ERα (siRNA SMARTpool ESR1), ERβ (siRNA SMARTpool ESR2), c-SRC (siRNA SMARTpool SRC), FAK (siRNA SMARTpool FAK), and control siRNAs (D-001810–01-05) were purchased from Dharmacon (Lafayette, CO). Gβ1 siRNAs, N-WASP, and Arp-2 siRNAs were from Santa Cruz Biotechnology (Santa Cruz, CA). The siRNAs were used at the final concentration of 50 nM. Breast cancer cells were treated 48 h after siRNAs transfection. Efficacy of gene silencing was checked with Western analysis and found to be optimal at 48 h.|
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