LIVE/DEAD™ Cell Imaging Kit

Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells

Experiment
Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells
Product
LIVE/DEAD™ Cell Imaging Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Add working solution and incubate for 15–30 min at 37 °C

Publication protocol

We used the Live/Dead Cell Imaging Kit (Molecular Probes, Life Technologies Corporation) to evaluate cell viability in our models. In the Live/Dead assay, the viable cells are stained green, while the dead cells are stained red35. The cells were loaded into the microfluidic devices, as described previously, and after one day, we used phosphate buffered saline (PBS, HyClone) to wash the culture chambers in the microfluidic chip for 1–3 min. Then, the cells were incubated with the Live/Dead Cell Imaging Kit for 15–30 min at 37 °C. Next, we used PBS again to wash out the reagent for 3–5 min and observed the culture chambers under a fluorescent microscope. Finally, cell viability was measured by assessing the percentage of green fluorescent cells in all of the cells.

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Papers

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Paper title
Microfluidic co-culture system for cancer migratory analysis and anti-metastatic drugs screening
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ Cell Imaging Kit below.

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