LIVE/DEAD™ Cell Imaging Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
The stains do not survive fixation or permeabilization.	Incubate cells at 37 °C for 4 h	Image quality may be improved by replacing media with Live Cell Imaging Solution

Upstream tips
The stains do not survive fixation or permeabilization.
Protocol tips
Incubate cells at 37 °C for 4 h
Downstream tips
Image quality may be improved by replacing media with Live Cell Imaging Solution

Publication protocol

LIVE/DEAD Cell Imaging Kit (Thermo Fisher Scientific) and Cell Count Reagent SF (Nacalai Tesque) were used, in accordance with the manufacturer’s instructions. Briefly, reagent was added to wells and the plate was incubated at 37 °C for 4 h. The optical density of formazan was detected at 450 nm by the GloMax-Multi Detection system (Promega) to calculate cell viability. A wavelength of 600 nm was used as a reference.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ Cell Imaging Kit below.

Download manufacturer protocol

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