Live-Dead cell staining kit (Enzo)

Live / Dead assay mammalian cells - human fibroblast tissue

Experiment
Live / Dead assay mammalian cells - human fibroblast tissue
Product
Live-Dead cell staining kit (Enzo) from Enzo Life Sciences
Manufacturer
Enzo Life Sciences

Protocol tips

Upstream tips
Mix 1 µL of Solution A and 1 µL of Solution B in 1 mL of Staining Buffer to make a staining solution
Protocol tips
Incubate for 15 minutes at 37°C.

Publication protocol

Primary human gingival fibroblasts, PDL cells and osteoblasts were seeded at a density of 10’000 cells per 24-well plate on control tissue culture plastic, with 20% conditioned media from PRP and 10% FBS. At 24 h post cell seeding, a live-dead staining assay was used to assess cell viability (Enzo Life Sciences AG; Lausen, Switzerland) as previously described [48]. Thereafter, the percentage of live versus dead cells was utilized to quantify cell viability.

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Papers

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Paper title
Effects of platelet rich plasma (PRP) on human gingival fibroblast, osteoblast and periodontal ligament cell behaviour
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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for Live-Dead cell staining kit (Enzo) below.

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