CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Cell cytotoxicity / Proliferation assay cell type - L-02

Experiment
Cell cytotoxicity / Proliferation assay cell type - L-02
Product
CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
- Completely thaw the 20ml size One Solution Reagent approximately 90 minutes at room temperature, or 10 minutes in a water bath at 37°C.
Protocol tips
- Cells were incubated with 20 μl of MTS for 3 h
Downstream tips
- To measure the absorbance later, add 25µl of 10% SDS to each well to stop the reaction. Store SDS-treated plates protected from light in a humidified chamber at room temperature for up to 18 hours.

Publication protocol

The cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay (CellTiter 96® AQueous One Solution Cell Proliferation assay, Promega, Madison, USA) as reported previously [7]. Briefly, cells were cultured in 96-well plates and treated with EGCG (0, 1.56, 3.125, 6.25, 12.5, 25, and 50 μM) for 2 h before being incubated in LPS (250 ng/ml) for another 48 h. Then, the treated cells were incubated with 20 μl of MTS for 3 h. The absorbance was measured at 490 nm with an automatic microplate reader (Tecan, Männedorf, Switzerland). Cell viability was calculated by the following formula: cell viability (fold) = the absorbance of treated group/the average absorbance of untreated group

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell cytotoxicity / Proliferation assay cell type - L-02 using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) from Promega.

Paper title
EGCG attenuates pro-inflammatory cytokines and chemokines production in LPS-stimulated L02 hepatocyte.
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Manufacturer protocol

Download the product protocol from Promega for CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) below.

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